Strain-related differences in immunosuppressive effects of Enterobacteriaceae and their lipopolysaccharides on production in rabbits of antibody to enterobacterial common antigen.

نویسندگان

  • H Y Whang
  • H Mayer
  • M G Schmidt
  • E Neter
چکیده

Certain polysaccharides have been shown to inhibit the antibody response of rabbits to the common enterobacterial antigen (CA). The present investigation revealed that striking differences exist in the immunosuppressive effects of enteric bacteria and their lipolysaccharides (lps), depending upon CA production by the strains. Mixtures of immunogenic strains (Escherichia coli F2378 [R4], E. coli F470 [R1], or Shigella boydii F3140 [R]) and non-immunogenic CA-producing strains, such as E. coli O1, E. coli O113, Salmonella montevideo, and S. minnesota, as well as the R mutants E. coli F614 (R1), E. coli F757 (R1), and S. typhimurium his 642 (Ra), failed to elicit CA antibodies. In contrast, mixtures of the immunogen and CA-negative strains S. typhimurium his 386 (Ra) and S. minnesota P595 (Re) or R555 (Ra) yielded antibodies in titers similar to those elicited by the immunogen alone. Further, LPS of CA-positive but not of CA-negative strains exerted this immunosuppressive effect. Quantitative studies revealed that LPS of S. minnesota in amounts of 100 mug/ml was strongly immunosuppressive, in amounts of 20 mug/ml slightly effective, and in amounts of 4 mug/ml ineffective. It is postulated that hitherto unknown differences exist, either in composition or in configuration, between LPS obtained from different microorganisms to account for the strain-related differences in immunosuppressive effects and, further, that the immunosuppressive LPS interacts with immunogenic CA.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Role of the rfa locus in the immunogenicity of common enterobacterial antigen.

Escherichia coli O14 is one of the few strains of Enterobacteriaceae which in the rabbit engender antibodies in high titers against the common enterobacterial antigen (CA). It is a rough strain that does not possess a specific side chain in its lipopolysaccharide structure (rfa+rfb-). An rfa mutant (rfa-rfb-) was isolated from this parental strain. Chemical analysis revealed the absence of one ...

متن کامل

Purification of human anti-erythropoietin polyclonal antibodies by precipitation and chromatography as an optimized method with potential application in vaccine studies

Introduction: Polyclonal antibodies are required to be affinity purified. Improved purification methods of polyclonal antibody provide an opportunity to pick the most purified immunoglobulins as a primary or secondary antibody in immunoassays that are included in many vaccine studies. Two common techniques for purifying proteins is salt precipitation and chromatography purification. Our work fo...

متن کامل

Effect of heat on antigenicity and immunogenicity of the antigenic determinant shared by Haemophilus influenzae type b and Escherichia coli K100.

Escherichia coli K100 produces an antigenic determinant similar to, or identical with, the capsular antigen of Haemophilus influenzae type b. Studies of the effects of heat on the immunogenicity, erythrocyte-modifying capacity, and antigenicity of this cross-reacting antigen (CRA) revealed the following findings. Immunization of rabbits with viable or formaldehyde-killed suspensions of E. coli ...

متن کامل

Preparation of antigens from midgut of Hyalomma anatolicum anatolicum and determining their immunoprotective effects

  Partially fed (4 -5 days) H .a. anatolicum (Iranian isolate) female adult ticks were used for preparation of antigens of midgut which are, the gut supernate antigen (GS), gut membrane antigen (GM), triton extracted gut membrane antigen ( TX-GM ) and sodium dodecyl sulphate treated Triton extracted gut membrane antigen (SDS-TX-GM). The New Zealand white rabbits were immunized with GS, GM, TX- ...

متن کامل

Production, Purification and Characterization of Chicken Egg Yolk Monoclonal Antibody Against Colonization factor antigen -1 of Enterotoxigenic Escherichia coli Causing Diarrhea

Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in both humans and animals. The contaminated food and water are the most common vehicles for ETEC infection. The colonization factor antigen (CFA-1) is a fimbriae protein that promotes adherence of the ETEC strain to the epithelium of the small intestine of the host. In this study IgY proteins were produced against the CFA-1 of ETEC in imm...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Infection and immunity

دوره 13 4  شماره 

صفحات  -

تاریخ انتشار 1976